Endogenous Topical Serum Preparations Retain Biological and Physiochemical Properties Following 6-Month Storage

Medical test tube with blood plasma in hands
Medical test tube with blood plasma in hands for PRP therapy, after centrifuge apparatus
Endogenous serum formulations may be used as a novel delivery system for skin regeneration in chronic diseases.

A novel storable and endogenous topical serum for cutaneous applications that is based on a patient’s own blood maintains both its biological and physicochemical properties after 6 months spent in storage, according to study findings published in the Journal of Cosmetic Dermatology.

Blood samples were taken from 6 healthy donors. The investigators collected 72 mL of peripheral blood into 9 mL collection tubes that contained 0.4 mL of 3.8% trisodium citrate as anticoagulant. The blood was then centrifuged and the whole plasma column was collected. The endogenous serum was dispensed into pump bottles and stored at 5°C±4°C for 6 months. Visual observation was used to examine organoleptic properties of endogenous serum samples. The investigators also examined microbiological contamination, spreadability index, and biological stability of endogenous serum.

According to findings from a hematologic analysis, the formulation was leukocyte-free and contained 1.93±0.03-fold platelet enriched plasma. At 6 months, the endogenous serum formulation had no odor and was slightly adherent to a vertical surface. An opaque yellow color was observed in the fresh and stored samples. There was a minimal increase in pH, from 7.55±0.06 to 7.99±0.08. By 6 months, the endogenous serum formulation was unaltered in regard to its organoleptic properties, viscosity, spreadability index, and sterility. Levels of insulin-like growth factor-I significantly decreased after 6 months (P <.05). No change was found in the proliferative activity in dermal fibroblasts.

Limitations of the study included its small sample size as well as the pre-clinical design.

Based on these findings, ES formulations may be used “as a novel delivery system to increase bioavailability and therapeutic effect of growth factors for skin regeneration,” which the researchers suggest could “reduce the frequency of blood extractions and would enable patients with chronic disorders to maintain a daily use of the product.”

Disclosure: Several study authors declared affiliations with the pharmaceutical industry. Please see the original reference for a full list of authors’ disclosures.


Anitua E, Pino A, Zalduendo M. Long-term stability of a novel platelet-rich plasma-based topical serum for cutaneous applications [published online July 12, 2020]. J Cosmet Dermatol. doi: 10.1111/jocd.13617